Serpent is a symmetric key block cipher that was a finalist in the Advanced Encryption Standard (AES) contest, where it was ranked second to Rijndael. Serpent was designed by Ross Anderson, Eli Biham, and Lars Knudsen.

Like other AES submissions, Serpent has a block size of 128 bits and supports a key size of 128, 192 or 256 bits. The cipher is a 32-round substitution–permutation network operating on a block of four 32-bit words. Each round applies one of eight 4-bit to 4-bit S-boxes 32 times in parallel. Serpent was designed so that all operations can be executed in parallel, using 32 bit slices. This maximizes parallelism, but also allows use of the extensive cryptanalysis work performed on DES.

Serpent took a conservative approach to security, opting for a large security margin: the designers deemed 16 rounds to be sufficient against known types of attack, but specified 32 rounds as insurance against future discoveries in cryptanalysis. The official NIST report on AES competition classified Serpent as having a high security margin along with MARS and Twofish, in contrast to the adequate security margin of RC6 and Rijndael (currently AES). In final voting, Serpent had the fewest negative votes among the finalists, but scored second place overall because Rijndael had substantially more positive votes, the deciding factor being that Rijndael allowed for a far more efficient software implementation.

The Serpent cipher algorithm is in the public domain and has not been patented. The reference code is public domain software and the optimized code is under GPL. There are no restrictions or encumbrances whatsoever regarding its use. As a result, anyone is free to incorporate Serpent in their software (or hardware implementations) without paying license fees.